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Re: DAB quantification using imageJ The usual question

Posted by ctrueden on Nov 04, 2014; 10:59pm
URL: http://imagej.273.s1.nabble.com/DAB-quantification-using-imageJ-The-usual-question-tp5010298p5010302.html

Hi Hesham,

> I'm in-need to measure area percentage and optical density if possible
> *can some one help me with clear steps*

Start here:
http://imagej.net/Segmentation

And see also:
http://www.researchgate.net/post/Any_good_methods_for_staining_quantifications_using_computer_programs

Regards,
Curtis

On Tue, Nov 4, 2014 at 12:10 PM, Dr. Hesham Noaman Abdelraheem Mustafa <
[hidden email]> wrote:

> Dear All
> I need an urgent help
> The usual question about DAB quantification using imageJ
> I have renal specimens and I used Desmin and caspase-3 immunohistochemistry
> markers.
> I'm in-need to measure area percentage and optical density if possible
> *can some one help me with clear steps*
>
> I think about imageJ, Fuji and Immunohistochemistry (IHC) Image Analysis
> Toolbox
>
> Waiting for your urgent contribution.
>
> --
>
>
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>
> *Dr. Hesham Noaman Abdelraheem MustafaAssistant Professor,Faculty of
> Medicine, King Abdulaziz University,e-Library Assistant,Student Support
> Unit Member,Nutrition & Metabolism Module Coordinator.Address: P.O.Box
> 80205               Jeddah, 21589               Saudi
> Arabia.Phone:           (Office):  +966 2 6400000 Ext. 22016
> (Mobile): +966 566 764 762           (Egypt):  +20 1001 460 009*
>
> *​Official Email: [hidden email] <[hidden email]>*
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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