Re: How to remove backround

Posted by Djoere Gaublomme on Nov 05, 2014; 2:34pm
URL: http://imagej.273.s1.nabble.com/How-to-remove-backround-tp5010271p5010316.html

Hi Mike,

Not 100% sure if I understand your question (I think it is: 'how can I  
only measure my ROI?'), and this might not be the best solution, but  
could you not just draw your ROI, then 'Edit' --> 'Clear Outside'  
before you measure?

grts

Djoere

Quoting Mike Tighe <[hidden email]>:

> ?I am trying to do some area measurements on lung tissue ( HandE  
> sections). I would like to be able to measure the total area of lung  
> tissue with the Background (empty spaces) subtracted. I can do this  
> very well using color threshold and the brightness sliders. I can  
> select to measure the area of background or the area of tissue and I  
> get very good results!
>
>
> I would then like to outline and measure area (ROI) of inflammation  
> that may have some "empty space" that needs to be subtracted. This  
> is where I am having a problem. I can't seem to be able to perform  
> the same function on an area as I can on the entire image.
>
>
> Anybody have any thought?
>
>
> Thanks for any help!
>
> Mike
>
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