Re: How does Multiview-Reconstruction compare to Zeiss?

Posted by Stephan Janosch on Nov 10, 2014; 9:35am
URL: http://imagej.273.s1.nabble.com/How-does-Multiview-Reconstruction-compare-to-Zeiss-tp5010290p5010351.html

Hey Mario!


> My biologists report that the DualSide Fusion with the Zeiss Zen
> Software is sometimes done while recording, but this only works for
> small Z-stacks for them, because for big stacks it takes the computer
> too long and imaging gets delayed. So I understand that this is a
> bottleneck for us - did you get this solved somehow?

Additionally to Pavels word I want to add a few details here:

We imagine C.elegans. We do Dual Side Illumination, which is not really
needed with our samples, but sometime the right side looks much better
or vice versa. I personally see the other illumination side as a
"backup". We did not understand yet, why we sometimes get different
imageging quality.

We also tried to do the online fusion with the Zen Software. (2014
Black). It worked without a problem, but the benefit was hardly visible.
Sometimes the results looked better, sometimes slightly worse.

After a few test sessions, we decided to do dual illumination, but not
to use the online fusion feature of the Zen software, because our
samples don't really benefit from it.

Best,
Stephan


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Stephan Janosch
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Max Planck Institute of Molecular Cell Biology and Genetics
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