Repeating ROI's in a cell

Posted by MichelleL on Nov 18, 2014; 10:34pm
URL: http://imagej.273.s1.nabble.com/Repeating-ROI-s-in-a-cell-tp5010534.html

Hello everyone,

I am very new to ImageJ, but have some programming experience in python, and was seeking some advice.  I am trying to quantify the change in intensity of a protein as a cell is migrating.  I would like to create a macro similar to the one in the image.  Basically, I would hand draw an ROI around a single cell.  Then run a macro that would repeat a predefined size hexagon within the boundaries of my pre-drawn ROI.  Then each ROI would record the mean intensities over 100-200 frames.  Then I would group that data by intensity and duration of the intensity change.

I am at a complete loss in how to do this.  I could program in the hexagon ROI, but how do I loop that ROI around the predefined limits of a cell?  Then, how do I get each ROI to record the intensity changes over time?

Any help at all would be much appreciated!

Thanks,

Michelle