Posted by
George Patterson on
Nov 19, 2014; 3:03pm
URL: http://imagej.273.s1.nabble.com/Repeating-ROI-s-in-a-cell-tp5010534p5010543.html
Hi Michelle,
I have a few questions.
>Basically, I would hand draw an ROI around a single
cell.
Will this be drawn for each time point? Or will you use the initial cell
outline for the entire series?
>Then run a macro that would repeat a predefined size hexagon within
the boundaries of my pre-drawn ROI. Then each ROI would record the mean
intensities over 100-200 frames.
Will the ROIs need to move as the cell moves? This relates to my first
question.
>Then I would group that data by intensity
and duration of the intensity change.
Just to be clear. Group by intensity in each frame? Or total intensity
over the course of the 100-200 frames?
George
On Tue, Nov 18, 2014 at 5:34 PM, MichelleL <
[hidden email]> wrote:
> Hello everyone,
>
> I am very new to ImageJ, but have some programming experience in python,
> and
> was seeking some advice. I am trying to quantify the change in intensity
> of
> a protein as a cell is migrating. I would like to create a macro similar
> to
> the one in the image. Basically, I would hand draw an ROI around a single
> cell. Then run a macro that would repeat a predefined size hexagon within
> the boundaries of my pre-drawn ROI. Then each ROI would record the mean
> intensities over 100-200 frames. Then I would group that data by intensity
> and duration of the intensity change.
>
> I am at a complete loss in how to do this. I could program in the hexagon
> ROI, but how do I loop that ROI around the predefined limits of a cell?
> Then, how do I get each ROI to record the intensity changes over time?
>
> Any help at all would be much appreciated!
>
> Thanks,
>
> Michelle
>
> <
http://imagej.1557.x6.nabble.com/file/n5010534/ImageJROI.png>
>
>
>
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