Sholl analysis composite and point selection issue

Posted by mia2005 on Nov 24, 2014; 8:51pm
URL: http://imagej.273.s1.nabble.com/Sholl-analysis-composite-and-point-selection-issue-tp5010602.html

Dear all,
I am trying to use the Sholl analysis plugin to batch analyze a group of images.

My images are originally in 16 bit color and they are z-stacked.

I start bye thresholding the image and convert it to binary format (0 or 255).

Then I select a point with the "point selection tool".

Then I start the Sholl analysis however, I continue having the same problem:

"Composite images are not supported" even though i set the threshold and I only have 0 or 255 pixels...

If I make a z-projection into a single plane I am able to run the Sholl analysis, however, I am not able to SAVE the point selection point into the file, even though I save it with Tif.

Does anyone has any suggestions for these problems?

1) how to run Sholl on z-projection

2) how to save a point done with the point selection tool

Thanks