Re: Objects Counter can't find any objects on my screen?

Posted by Thomas Boudier on Nov 26, 2014; 2:52am
URL: http://imagej.273.s1.nabble.com/Objects-Counter-can-t-find-any-objects-on-my-screen-tp5010585p5010628.html

Dear Elliot,

It seems taht your image is 16 bits but signed, with negative values, so
threshold algorithm may not work properly with this kind of data. I then
advise you to :

1/ duplicate your original image

2/ convert the copy to 8-bits

3/ use segmentation from 3DManager

4/ add the segmented image to 3DManager

5/ select original image and perform quantification

Hope this helps

Thomas


On 25/11/14 19:31, esumft wrote:

> I must add, if I convert the image to 8-bit grayscale it works. But, it
> changes the values of the intensity (this is calibrated with a DICOM header)
> so the readings I get from the 3DObjectsDetector will be wrong?
>
>
>
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      Thomas Boudier, Associate Professor, UPMC,
      Université Pierre et Marie Curie, Paris, France.
      BioInformatics Institute (BII)/IPAL, Singapore.
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