DeconvolutionLab in ImageJ (Fiji)

Posted by bcli on Dec 01, 2014; 2:43am
URL: http://imagej.273.s1.nabble.com/DeconvolutionLab-in-ImageJ-Fiji-tp5010704.html

Hi all!

I am currently in the early stages of implementing deconvolution to images acquired through epi-fluorescence microscopy.

I have imaged subresolution beads that can be used as point spread functions that describe the diffraction patterning under its specific image acquisition conditions (wavelength etc). Theoretically, I should be able to use an averaged PSF and deconvolve a single subresolution bead and reconstruct it into a bead of higher resolution (without the diffraction blur). Image dimensions and Z steps are all the same for my images (Z steps enough to visualize clear diffraction blur).

I have mainly been using the Richardson-Lucy algorithm in DeconvolutionLab, but my images have been coming out identical to my input image. I ensured the inputted images are correct as well, and I believe I have tried close to everything with respect to fiddling with deconvolutionlab. Perhaps it is a problem intrinsic to the bead images collected?

Has anyone else encountered the same problem?



Appreciate the input and thank you in advance!

Warmly,
Brian