Manual registration of two images

Posted by David Strachan on Dec 03, 2014; 1:24pm
URL: http://imagej.273.s1.nabble.com/Manual-registration-of-two-images-tp5010738.html

Hi,

I am trying to find a plugin to allow registration of two images (RGB) but to do this manually. The two images are serial sections with different staining and so it is not that easy to see the common points in each image. I was hoping to find something that could show one image with transparent background overlaid on the other image and then allow me to manually move in x and y dir and rotation until I have them overlaid.

There seems to be lots of options preinstalled in FIJI (all with no instructions so although it may do exactly what I want I don't know) I have tried faffing around with these but time is of the essence, I need the analysis results by this Friday.

There was one plugin that sound the most promising in that I draw a line in one image between two points and then draw a line in the other image between the same tow landmark points and then it came up with loads of errors as It was then trying to do something automatically and the images are not similar enough.

Does anyone know if a macro where I can either interactively move one image over another to align or alternatively select 2 points in one image then select the matching two points in the other image and then get these images to orientate themselves to be the same.

I do not want the image to be overlaid permanently as I want to use the staining on one mage as a mask for subsequent measurement on the second image.


David Strachan

Beatson Institute for Cancer Research
Garscube Estate,
Switchback Road,
Glasgow, G61 1BD
Scotland
U.K.

Tel [phone]


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