Posted by
Christian Goosmann-2 on
Dec 03, 2014; 2:02pm
URL: http://imagej.273.s1.nabble.com/Manual-registration-of-two-images-tp5010738p5010739.html
Dear David
you could use the TrakEM 2 plugin, generate a project with 2 z-planes,
import one of your images in each plane. It is a bit of a learning curve
but doable till Friday, there are good tutorials. You can set the first
plane so it blends into your second plane (control the transparency in
'Layers' tab) and then manually shift the image in the second plane
around until it fits. From the project you can export a stack of both
images with image two translated/rotated in the step before. I can't
tell you all controls, but most things in TrakEM 2 work by using the
context menus you get with right-click. TrakEM2 is included in FIJI.
Hth
Christian
---
Christian Goosmann
Mikroskopie
Max-Planck-Institut für Infektionsbiologie
Campus Charité Mitte
Charitéplatz 1
10117 Berlin
Tel.: +49 30 28460 388
David Strachan wrote:
> Hi,
>
> I am trying to find a plugin to allow registration of two images (RGB) but to do this manually. The two images are serial sections with different staining and so it is not that easy to see the common points in each image. I was hoping to find something that could show one image with transparent background overlaid on the other image and then allow me to manually move in x and y dir and rotation until I have them overlaid.
>
> There seems to be lots of options preinstalled in FIJI (all with no instructions so although it may do exactly what I want I don't know) I have tried faffing around with these but time is of the essence, I need the analysis results by this Friday.
>
> There was one plugin that sound the most promising in that I draw a line in one image between two points and then draw a line in the other image between the same tow landmark points and then it came up with loads of errors as It was then trying to do something automatically and the images are not similar enough.
>
> Does anyone know if a macro where I can either interactively move one image over another to align or alternatively select 2 points in one image then select the matching two points in the other image and then get these images to orientate themselves to be the same.
>
> I do not want the image to be overlaid permanently as I want to use the staining on one mage as a mask for subsequent measurement on the second image.
>
>
> David Strachan
>
> Beatson Institute for Cancer Research
> Garscube Estate,
> Switchback Road,
> Glasgow, G61 1BD
> Scotland
> U.K.
>
> Tel +44 141 330 6872
>
>
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