Posted by
CARL Philippe (LBP) on
Dec 03, 2014; 2:15pm
URL: http://imagej.273.s1.nabble.com/Manual-registration-of-two-images-tp5010738p5010740.html
Dear David,
In order to make a manual registration, just make a color channel
(Image->Color->Merge_Channels...) where you put one picture in red and the
other in green.
Then you select all (Ctrl + A), copy one channel (Ctrl + C), paste it (Ctrl
+ V) and then you can drag a channel with the arrow keys with respect to the
other one.
It is straight forward and working quite well...
Nevertheless you have only a precision of +/- 1 pixel and you can't go below
with this method.
So now I use either the StackReg or JavaSIFT plugins and they are making the
job much easier...
My best regards,
Philippe
Philippe CARL
Laboratoire de Biophotonique et Pharmacologie
UMR 7213 CNRS - Université de Strasbourg
Faculté de Pharmacie
74 route du Rhin
67401 ILLKIRCH
Tel : +33(0)3 68 85 41 84
-----Message d'origine-----
De : ImageJ Interest Group [mailto:
[hidden email]] De la part de David
Strachan
Envoyé : mercredi 3 décembre 2014 14:24
À :
[hidden email]
Objet : Manual registration of two images
Hi,
I am trying to find a plugin to allow registration of two images (RGB) but
to do this manually. The two images are serial sections with different
staining and so it is not that easy to see the common points in each image.
I was hoping to find something that could show one image with transparent
background overlaid on the other image and then allow me to manually move in
x and y dir and rotation until I have them overlaid.
There seems to be lots of options preinstalled in FIJI (all with no
instructions so although it may do exactly what I want I don't know) I have
tried faffing around with these but time is of the essence, I need the
analysis results by this Friday.
There was one plugin that sound the most promising in that I draw a line in
one image between two points and then draw a line in the other image between
the same tow landmark points and then it came up with loads of errors as It
was then trying to do something automatically and the images are not similar
enough.
Does anyone know if a macro where I can either interactively move one image
over another to align or alternatively select 2 points in one image then
select the matching two points in the other image and then get these images
to orientate themselves to be the same.
I do not want the image to be overlaid permanently as I want to use the
staining on one mage as a mask for subsequent measurement on the second
image.
David Strachan
Beatson Institute for Cancer Research
Garscube Estate,
Switchback Road,
Glasgow, G61 1BD
Scotland
U.K.
Tel +44 141 330 6872
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