Posted by
Malgorzata Swider on
Dec 03, 2014; 2:30pm
URL: http://imagej.273.s1.nabble.com/Manual-registration-of-two-images-tp5010738p5010741.html
Hi David,
you can try a "Template Matching'" plugin.
it allows you to select a rectangle region as a landmark on a reference
image.
sometimes it works pretty well, depending on images.
Best,
Gosia
------------------------------------------------
Malgorzata Swider, Ph.D.
Research Specialist
Scheie Eye Institute, University of Pennsylvania
51 North 39th St, Philadelphia PA 19104, USA
Ph: (215) 662-8043
--------------------------------------------------
From: "David Strachan" <
[hidden email]>
Sent: Wednesday, December 03, 2014 8:24 AM
To: <
[hidden email]>
Subject: Manual registration of two images
> Hi,
>
> I am trying to find a plugin to allow registration of two images (RGB) but
> to do this manually. The two images are serial sections with different
> staining and so it is not that easy to see the common points in each
> image. I was hoping to find something that could show one image with
> transparent background overlaid on the other image and then allow me to
> manually move in x and y dir and rotation until I have them overlaid.
>
> There seems to be lots of options preinstalled in FIJI (all with no
> instructions so although it may do exactly what I want I don't know) I
> have tried faffing around with these but time is of the essence, I need
> the analysis results by this Friday.
>
> There was one plugin that sound the most promising in that I draw a line
> in one image between two points and then draw a line in the other image
> between the same tow landmark points and then it came up with loads of
> errors as It was then trying to do something automatically and the images
> are not similar enough.
>
> Does anyone know if a macro where I can either interactively move one
> image over another to align or alternatively select 2 points in one image
> then select the matching two points in the other image and then get these
> images to orientate themselves to be the same.
>
> I do not want the image to be overlaid permanently as I want to use the
> staining on one mage as a mask for subsequent measurement on the second
> image.
>
>
> David Strachan
>
> Beatson Institute for Cancer Research
> Garscube Estate,
> Switchback Road,
> Glasgow, G61 1BD
> Scotland
> U.K.
>
> Tel +44 141 330 6872
>
>
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