Re: Is manual thresholding methods accepted by scientific journals?

Posted by Василий Попков on Dec 06, 2014; 6:53pm
URL: http://imagej.273.s1.nabble.com/Is-manual-thresholding-methods-accepted-by-scientific-journals-tp5010814p5010821.html

I mean just to take an image of something what is "background" in general.
And than use this value to minus it from all image and count it like
thresholding. Depends on your object, I guess.

As for me, I have tested all thresholding methods and only Shanbhag works
quite well for me. Still, manual method was better :(

For now I am doing so: find manually threshold for 1 image and than use
exactly the same values for all images in experiment. My objects are
supposed to have the same noise and background levels in 1 experiment.

2014-12-06 21:26 GMT+03:00 MrScienctistMan (Original poster) <
[hidden email]>:

> I guess for example the triangle method in ImageJ looks pretty okay. Would
> it be more aceptable to use for example this instead of manual threshold?
>
> Im not quite sure what you mean about empty image. Do you mean a
> non-stained negative control?
>
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>

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