count nuclei in a volume

Posted by Cesar Mendes on Dec 08, 2014; 7:08pm
URL: http://imagej.273.s1.nabble.com/count-nuclei-in-a-volume-tp5010848.html

Greetings,

I want to count the number of nuclei in a tissue which express a nuclear marker (H2B:YFP). Is there a good way to do this automatically?
Attached you can see a stack of one of these images. This is a low populated example, i have other genotypes that will be much more crowded and harder to count manually.
I acquire data from a Leica confocal that can be converted to imageJ stacks.
Some cells have a decreased level of the marker and that might be an issue.
Thanks in advance!
Best,
Cesar



 
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