Re: Is manual thresholding methods accepted by scientific journals?

Posted by Василий Попков on Dec 09, 2014; 5:29am
URL: http://imagej.273.s1.nabble.com/Is-manual-thresholding-methods-accepted-by-scientific-journals-tp5010814p5010857.html

Hi.

As for me I think you have no other options, but to use one of auto thr.
methods. But the same for all 1000 images.

2014-12-09 6:24 GMT+03:00 giuseppe3 <[hidden email]>:

> Hi everyone,
>
> just to make things more confusing, I am writing a macro which segments
> yeast cells from >10000 images. Since these images do not all have the same
> number of cells (can vary from 50 to 500 cells per image) and where
> obtained
> using slightly different microscope settings I need to change the threshold
> between images to get the maximum possible number of cells from each.
>
> Do you think this is reasonable? I am only trying to get the total cell
> number.
>
> Thanks
>
>
>
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