Re: analyze different marked cells

Posted by Joel Sheffield on Dec 11, 2014; 6:04pm
URL: http://imagej.273.s1.nabble.com/analyze-different-marked-cells-tp5010893p5010909.html

Hi Jorg,

I am as puzzled as the others about which criteria you are using to
discriminate between the two kinds of labeling.  As I see it, it appears
that the cells that you do not want to count have a large amount of
staining, that fills the nucleus, and the ones that you want to count have
much smaller regions of stain.  Those regions, however, appear to be
contiguous --that is they are not composed of small spots.  So, I carried
out a color threshold on the original image, selecting only on hue, and was
able to see the populations of cells with large nuclei, and those with
small regions of stain. Note that I kept the brightness and saturation
settings wide open.  I assume that I could then isolate the ones that I
care about by selecting a size criterion in the Analyze Particles routine.
Or is this not what you had in mind?

Joel[image: Inline image 1]Note, I k



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  *http://tinyurl.com/khbouft <http://tinyurl.com/khbouft>*

On Thu, Dec 11, 2014 at 4:48 AM, Jörg <[hidden email]>
wrote:

> Hello everyone,
>
> I try to discriminate between different marked cells (see attached). so i
> first substracted the background and then did a color threshold. but now i
> have the problem that there are not only the marked cells i want to count
> but also some edges of the other cells (2nd attachement). is there any way
> to get rid of them?
>
> best,
> jörg
>
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