Multinucleate automatic cell counting?

Posted by Monkey on
URL: http://imagej.273.s1.nabble.com/Multinucleate-automatic-cell-counting-tp5011080.html

I am trying to automate image analysis for high-throughput scoring of cells, where I would like to extract the percentage of multinucleate versus mononucleate cells.  I have used the BioVoxxel plugin to select cell bodies (as objects) from one binary image layer using a DAPI nuclear stain binary image layer (as selector) to weed out dead cells.  

This worked well, but now I want extract information about multinuclearity:

    1)  How many nuclei (from binary DAPI image) overlap with the cell body (from binary cell body image, TRITC channel, labeling cells of interest in contrast to other cells in a co-culture)

    2)  For each overlapping nucleus, does it lie on the border of the cell body (since these may not be a part of the cell of interest, and I will have to either manually check them or work out an automated method to exclude the possibility they are from a different type of cell than the one I am interested in)?

I imagine it would not be too difficult to code, but I was wondering if anyone knows of a plugin that can already do this?

Many thanks for any help!

Justin