Posted by
Monkey on
Jan 05, 2015; 9:28pm
URL: http://imagej.273.s1.nabble.com/Multinucleate-automatic-cell-counting-tp5011083.html
I am trying to automate image analysis for high-throughput scoring of cells,
where I would like to extract the percentage of multinucleate versus
mononucleate cells. I have used the BioVoxxel plugin to select cell bodies
(as objects) from one binary image layer using a DAPI nuclear stain binary
image layer (as selector) to weed out dead cells.
This worked well, but now I want extract information about multinuclearity:
1) How many nuclei (from binary DAPI image) overlap with the cell body
(from binary cell body image, TRITC channel, labeling cells of interest in
contrast to other cells in a co-culture)
2) For each overlapping nucleus, does it lie on the border of the cell
body (since these may not be a part of the cell of interest, and I will have
to either manually check them or work out an automated method to exclude the
possibility they are from a different type of cell than the one I am
interested in)?
I imagine it would not be too difficult to code, but I was wondering if
anyone knows of a plugin that can already do this?
Many thanks for any help!
Justin
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