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Re: Multinucleate automatic cell counting?

Posted by Robert Baer on Jan 06, 2015; 1:43am
URL: http://imagej.273.s1.nabble.com/Multinucleate-automatic-cell-counting-tp5011083p5011085.html

You might take a look at cell profiler for this type of high throughput
task: http://www.cellprofiler.org/

Rob
On 1/5/2015 3:28 PM, Monkey wrote:

> I am trying to automate image analysis for high-throughput scoring of cells,
> where I would like to extract the percentage of multinucleate versus
> mononucleate cells.  I have used the BioVoxxel plugin to select cell bodies
> (as objects) from one binary image layer using a DAPI nuclear stain binary
> image layer (as selector) to weed out dead cells.
>
> This worked well, but now I want extract information about multinuclearity:
>
>      1)  How many nuclei (from binary DAPI image) overlap with the cell body
> (from binary cell body image, TRITC channel, labeling cells of interest in
> contrast to other cells in a co-culture)
>
>      2)  For each overlapping nucleus, does it lie on the border of the cell
> body (since these may not be a part of the cell of interest, and I will have
> to either manually check them or work out an automated method to exclude the
> possibility they are from a different type of cell than the one I am
> interested in)?
>
> I imagine it would not be too difficult to code, but I was wondering if
> anyone knows of a plugin that can already do this?
>
> Many thanks for any help!
>
> Justin
>
>
>
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