Posted by
G. Esteban Fernandez on
Jan 06, 2015; 4:47am
URL: http://imagej.273.s1.nabble.com/Integrated-Pixel-Density-Units-tp5011086p5011095.html
Hi Hazel,
The decimal numbers you gave take into account the spatial scaling (pixel
size) of the image. For a strict sum of pixel intensities (without regard
to spatial scaling) use the Raw Integrated Density (RawIntDen) value, which
will be a whole number.
To see/test this for yourself, change the scaling of an image (Image >
Properties) to 1.0 x 1.0 pixels and your IntDen will match RawIntDen. If
you change the pixel scaling to, for example, 0.1 x 0.1 microns your IntDen
will be 1/100th of the RawIntDen value.
To answer your original question, I guess the units of IntDen are 1/unit^2
(e.g. 1/micron^2).
-Esteban
On Jan 5, 2015 8:24 PM, "May, Hazel - (hazelmay)" <
[hidden email]> wrote:
> Dear Sir/Madam
>
> I am having trouble establishing the units of integrated Pixel Density.
> So far I have analysed an 8-bit image with subtracted background and has a
> threshold of 0-255 of silver stained rat brain tissue.
> I know so far that pixel density is the sum of all the pixels in the given
> analysed area...
> But what are the units? When you get results summary, you get at 0.8 or
> 0.988 or 0.33 or even 1.23 - but in what?
>
> Many thanks,
> Hazel May
>
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