Posted by
MODEL, MICHAEL on
Jan 06, 2015; 6:03pm
URL: http://imagej.273.s1.nabble.com/Integrated-Pixel-Density-Units-tp5011086p5011097.html
Hazel,
Your Min and Max are the same, are measuring a single pixel?
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of May, Hazel - (hazelmay)
Sent: Tuesday, January 06, 2015 12:15 PM
To:
[hidden email]
Subject: Re: Integrated Pixel Density Units
Thank you,
Another question however; my images were in inches as it appears. I have changed this to microns Now values are huge – due to the conversion obviously
I also changed the scaled of the pixel width and height. To 1.0 and 0.1 microns and this alters my original values.
If I were to graphy my results would and now I have gigantic numbers
With inches and I have done analysis I get these results summary:
Slice Count total area avg size %area mean IntDen
362686_30_RIGHT_PAAP_SILVER_20X-1.tif 16004 49.323 0.003 9.086 137.340 0.378
However in the results I get:
Area mean StdDev Min Max IntDen %Area RawIntDen
16004 1.085E-4 144 0 144 144 0.016 100 144
Those are all when the images are in inches
Now when I have them in Microns
The Summary
Slice Count total area avg size %area mean IntDen
362686_30_RIGHT_PAAP_SILVER_20X-1.tif 16004 47103308932.719 2943221.003 9.086 137.340 360729529.321
Results
Area mean StdDev Min Max IntDen %Area RawIntDen
16004 103623.280 144 0 144 144 14921752.276 100 144
Im Struggling to interpret the results here I was told to take the IntDen from the summary and not the results…but they ‘re different numbers Plus I am not following the 1/unit^2 means..
Say unit being inches or microns. Is it a specific measurement of either unit?
Sorry If I am asking basic questions or not being clear.
I Just don’t know what I don’t know.
Many Many Thanks
Hazel
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Michael Sarahan
Sent: Monday, January 05, 2015 9:57 PM
To:
[hidden email]
Subject: Re: Integrated Pixel Density Units
Hi Hazel,
These numbers may express the percentage of integrated intensity represented by any given region. Do you have something on the order of 100 regions in your image? The tutorial at:
http://imagej.nih.gov/ij/docs/examples/dot-blot/ suggested this, which I found by looking at the Integrated Density entry at:
http://rsbweb.nih.gov/ij/docs/guide/146-30.htmlI hope this helps.
Michael
On Mon, Jan 5, 2015 at 7:51 PM, May, Hazel - (hazelmay) <
[hidden email]> wrote:
> Dear Sir/Madam
>
> I am having trouble establishing the units of integrated Pixel Density.
> So far I have analysed an 8-bit image with subtracted background and
> has a threshold of 0-255 of silver stained rat brain tissue.
> I know so far that pixel density is the sum of all the pixels in the
> given analysed area...
> But what are the units? When you get results summary, you get at 0.8
> or
> 0.988 or 0.33 or even 1.23 - but in what?
>
> Many thanks,
> Hazel May
>
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