Re: Analysing water in oil droplets

Posted by gankaku on Jan 08, 2015; 6:17pm
URL: http://imagej.273.s1.nabble.com/Analysing-water-in-oil-droplets-tp5011113p5011133.html

Hi Rikschuff, hi Michael,

the following macro gives a very similar result but should not have
problems with the background nor the different shading. One part is based
on the Difference of Gaussian and this should make the outlines of your
droplets here more pronounced. But this currently also just solves the
detection step and still not the tracking part.

//------------------------------------------
roiManager("reset");
setBatchMode(true);
setOption("BlackBackground");
original = getTitle();
run("8-bit");
run("Invert");
selectWindow(original);

run("Invert");
sig1 = 0.5;
sig2 = 3;
run("Duplicate...", "title=DoG");
DoG = getTitle();
run("Duplicate...", "title=intermediate");
intermediate = getTitle();

selectWindow(intermediate);
run("Gaussian Blur...", "sigma=sig1");
selectWindow(DoG);
run("Gaussian Blur...", "sigma=sig2");

imageCalculator("Subtract", "DoG", "intermediate");

selectWindow(DoG);

run("Enhance Contrast...", "saturated=1 normalize");
run("Auto Threshold", "method=Huang white");
run("Fill Holes");

run("Analyze Particles...", "size=500-Infinity circularity=0.80-1.00
show=Masks display exclude clear summarize add");
setBatchMode(false);
run("Invert LUT");
//------------------------------------------

Hope it helps.
cheers,
Jan

2015-01-08 10:53 GMT+01:00 rikschuff <[hidden email]>:

> Mm that's strange :) Thanks for noticing. Here is the image:
> <http://imagej.1557.x6.nabble.com/file/n5011120/DSC_0758_-_Copy.jpg>
>
>
>
> --
> View this message in context:
> http://imagej.1557.x6.nabble.com/Analysing-water-in-oil-droplets-tp5011113p5011120.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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