Counting nuclei without making image binary

Posted by KerL on
URL: http://imagej.273.s1.nabble.com/Counting-nuclei-without-making-image-binary-tp5011196.html

Dear all,

I am trying to use ImageJ/Fiji to count cells stained for their nuclei that are only surrounded by a different stain. As such, I do not believe making the image binary will be the best option for me, as it will count all the cells, even the ones without the stain surrounding it.
I have tried the plugin "Nucleus Counter" as an option but I have found that it uses 16-/8-bit images and therefore I cannot adjust for the color threshold. Also the shape of the cells I am counting are irregular and this plugin often mistakes 1 cell as 2 cells etc..
 
Is there a different way of going about this task?

Thank you in advance,

Erong