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Re: Touching particle accurate counting

Posted by Adam Hughes on Jan 14, 2015; 8:04pm
URL: http://imagej.273.s1.nabble.com/Touching-particle-accurate-counting-tp5011214p5011221.html

Is it possible to do some sort of geometric averaging and estimate the
count?  For example, if you measure a composite particle that has 3 times
the average area (which you'd set I presume), then it bins this as 3
particles.  Or do you need the exact measurements of every single particle?

On Wed, Jan 14, 2015 at 2:46 PM, Al Menia <[hidden email]> wrote:

> Hey! Thanks for answering.
>
> This is a backscattered image, but this SEM seems to add a little SE
> detection, which indeed add shadows in the image (I will use another SEM or
> change some parameters next time). I normally have .tif images but it seems
> that this website does not accept them, so  I had to convert them in .png.
>
> Here is are two other .jpg images with no shadows (and no white particles
> as
> you called them), I still have a lot of difficulties to process them
> properly... Having a global idea of the number of diamonds is all right,
> but
> if i want to have 1% accuracy or so it's another problem.
>
> I added the processed images to illustrate the problem: some particles are
> split with watersheding and become too small, whereas some others are not
> cut and are too big. Is there a way to fix that?
> Image 1 <http://i.imgur.com/IkOeq0C.jpg>
> image 1 processed <http://i.imgur.com/2MEaKvm.jpg>
> image 2 <http://i.imgur.com/HQFwcL0.jpg>
> image 2 processed <http://i.imgur.com/KHcwR3O.jpg>
>
> What really annoys me is that the human eye perfectly count the diamonds,
> but that it becomes incredibly more difficult to do it with a computer :(
>
>
>
>
>
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