Threshold methods, how to choose suitable one?

Posted by Sarah Mohamed on Jan 15, 2015; 5:49pm
URL: http://imagej.273.s1.nabble.com/Threshold-methods-how-to-choose-suitable-one-tp5011239.html

Hi

I would like to ask about the suitable choice for threshold method to quantify the area fraction of brown color.

I have H/DAB images for treated groups. I need to use a method that is least subjective, gives consistent results meaning if i use it several times gives the exact same area fraction.

There are several methods:

1, Image>>adjust>>threshold, moving brightness and hue sliders to get 2 areas then divide them to get area fraction.

2. Image>>color>>split. choose green chanel. Image>>adjust>>threshold>>clt+M

3. www.med.upenn.edu/cellbio/documents/imagej_colorsegmentpdf.pdf

Image.. Plugins..threshold color...move saturation slider to select ROI..close plugin..image..type...8bits..image..adjust...t hreshold..clt+M.

I know there may be other methods for color segmentation but thats all i know. I cant use color deconvolution as i cant do background correction calculation ...i am just a pharmacologist.

Thanks in advance.
Sarah M.Mosaad, M.Sc.

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