Re: problems with the function: analyse particles

Posted by AaronUCF2014 on Jan 15, 2015; 11:15pm
URL: http://imagej.273.s1.nabble.com/problems-with-the-function-analyse-particles-tp4999891p5011243.html

If you change to an 8-bit image or RGB stack, this will change.
You can play around with inverting the image and then converting to an RGB stack and then setting a threshold. Then making an ROI and measuring to get %Area, which gives the percent of that area meeting the defined threshold.
If all images are acquired in the exact same manner and then processed in this manner with the same threshold set, it should give relatively quantitative measurements.