unit for surface value

Posted by Muriel Desbois on Jan 19, 2015; 7:19pm
URL: http://imagej.273.s1.nabble.com/unit-for-surface-value-tp5011285.html

Hi,

I would like to know at is the unit for the surface value given using the ROI Manager.

I'm trying to get the surface value of synapses. I have an in vivo fluorescent marker for synapses. I take a z-stack of the signal, then project the maximum intensity to have a single image. Then I circled the different dots and extract the different values using the ROI Manager. For the surface I have value between 0.0001 and 0.001. I'm trying to understand what is the unit of this "surface". I read it is in square pixel but my issue is how the surface can be inferior to 1.

Thank you very much for your help

Muriel

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