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Re: Threshold methods, how to choose suitable one?

Posted by Sarah Mohamed on Jan 20, 2015; 9:04pm
URL: http://imagej.273.s1.nabble.com/Threshold-methods-how-to-choose-suitable-one-tp5011232p5011303.html

Hi Cameron,
Thanks for your reply.

In order to do color deconvolution i must do background correction & enter my own vectors; my knowledge in imaging is little, i find difficulty to go through all these steps (bright field, dark field calculation,..etc).

so  i am trying to find another segmentation method that is consistent and not subjective.

Regards,
Sarah M. Mosaad, M.Sc.

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