Re: Coloc2 issues

Posted by Tyagi, Ayushi on Jan 22, 2015; 9:16pm
URL: http://imagej.273.s1.nabble.com/Coloc2-issues-tp5011257p5011316.html

Hello Curtis,

I have successfully downloaded a new version of fiji that properly opens
up coloc 2. I am getting some warnings stating that the threshold for my
images is too high and that the y-intercept is high. There are some
suggestions on how to fix that issue but I have already selected an ROI
and it does not get rid of the warnings. I subtracted background for both
images before running coloc 2 as well.

Thank you for your help thus far,

Ayushi

On 1/21/15 12:47 PM, "Curtis Rueden" <[hidden email]> wrote:

>Hi Ayushi,
>
>> Would it be easier to uninstall fiji and reinstall an updated version?
>
>Yes. If you are having problems with the updater, downloading a new copy
>of
>Fiji is the simplest workaround.
>
>If the problem persists after that, definitely speak up.
>
>Regards,
>Curtis
>
>On Tue, Jan 20, 2015 at 2:09 PM, Tyagi, Ayushi <
>[hidden email]> wrote:
>
>> Hey Curtis,
>>
>> I have tried to update fiji many times and an error message pops up
>>saying
>> the file was not found. Would it be easier to uninstall fiji and
>>reinstall
>> an updated version?
>>
>> Ayushi
>>
>> On 1/16/15 3:54 PM, "Curtis Rueden" <[hidden email]> wrote:
>>
>> >Hi Ayushi,
>> >
>> >> I have installed the coloc2 plugin into fiji
>> >
>> >Fiji comes with the Coloc 2 plugin preinstalled; you should not have to
>> >install anything else.
>> >
>> >The plugin ostensibly works for me with an up-to-date Fiji.
>> >
>> >Regards,
>> >Curtis
>> >
>> >On Fri, Jan 16, 2015 at 1:12 PM, Tyagi, Ayushi <
>> >[hidden email]> wrote:
>> >
>> >> Hello,
>> >>
>> >> I have installed the coloc2 plugin into fiji however, if I try to use
>> >> coloc 2 a window pops up that says
>> >>
>> >>
>>
>>>>/Applications/Fiji.app/plugins/Colocalisation_Analysis-master/src/main/
>>>>ja
>> >>va/Coloc_2.java:1:
>> >> package algorithms does not exist
>> >> import algorithms.Algorithm;
>> >>
>> >> I have split the image into separate channels so I do not think that
>>is
>> >> the issue.
>> >>
>> >> Please let me know what other information you need.
>> >>
>> >> Best,
>> >>
>> >> Ayushi
>> >>
>> >>
>> >>
>> >> --
>> >> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>> >>
>> >
>> >--
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>>
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>
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