Re: registering hyper-spectral stacks
Posted by
Gabriel Landini on
Jan 30, 2015; 9:41am
URL: http://imagej.273.s1.nabble.com/registering-hyper-spectral-stacks-tp5011376p5011385.html
On Wednesday 28 Jan 2015 09:41:58 you wrote:
> I have a bunch of stacks of images. About 1500x1500, 16 bit grayscale.
> Each image in the stack represents a small slice of the visible light
> spectrum (about 25 images per stack, more or less).
>
> Actually, there are TWO stacks covering the spectrum - leading to two
> “composite” images - but I think we can ignore that for now.
>
> My question is: has anyone used one of the “stack registration” tools to
> register this type of image. Most of the demos I see imply that the stack
> registration tools are intended for stacks which move in Z through a
> volume. My stacks are all of the same field of view (well…almost…and
> that’s the problem) and move through the spectrum.
I used the "Linear Stack Alignment with SIFT" just once and it did work OK on
a stack of multispectral slices.
http://fiji.sc/Linear_Stack_Alignment_with_SIFTCheers
Gabriel
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