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Re: problem with ND2 reader in latest Bio-Formats in Fiji

Posted by Aryeh Weiss on Feb 02, 2015; 6:05pm
URL: http://imagej.273.s1.nabble.com/Latest-Bio-Formats-in-Fiji-tp5009834p5011412.html

Hi Curtiss

I uploaded an rather large file when I first reported this. Since the
files are quite large  (2-4GB), please let me know if you still have
that one before I upload another one. I cannot do an upload of that size
from home.

Best regards
--aryeh

On 2/2/15 7:35 PM, Curtis Rueden wrote:

> Hi Aryeh,
>
> > The latest update of Bio-formats still does not read my multifield ND2
> > files correctly.
>
> Very sorry to hear that there are still problems reading your data.
>
> I spoke with Melissa briefly about this issue. The OME developers will
> need a sample non-working dataset in order to debug the issue. The
> best place to follow up is via the OME support channels:
>
> - http://lists.openmicroscopy.org.uk/mailman/listinfo/ome-users/
> - http://qa.openmicroscopy.org.uk/qa/upload/
>
> Regards,
> Curtis
>
> On Mon, Feb 2, 2015 at 10:48 AM, Aryeh Weiss <[hidden email]
> <mailto:[hidden email]>> wrote:
>
>     On 2/2/15 5:00 PM, Knecht, David wrote:
>
>         Hi Mark- Has there been any progress on opening ND2 files with
>         multiple positions?  I suggested a “split positions” checkbox
>         a while back, but that seems to not have been implemented.  
>         If there is no Z, the system interprets positions as z
>         slices.   At this point, I don’t know a way to open
>         multichannel, multi-z, multi position datasets other than to
>         split them in Elements. Dave
>
>         On Feb 2, 2015, at 9:51 AM, Mark Hiner <[hidden email]
>         <mailto:[hidden email]>> wrote:
>
>
>     Hi David
>
>     You can open multifield ND2 files with bioformats. What you need
>     to maek it work correctly is the formats-gpl05.0.2.jar file, which
>     still works correctly with the 5.0.6 distribution. After you
>     insert that, you should make sure that it does not get updated
>     when you update Fiji.
>
>     I can send it to you offlist if you want it. Insert it into
>     jars/bioformats , and make sure to remove the existing file.
>
>     With this workaround, I opened a file with 20 fields, each of
>     which has 3 channels an 17 z-positions.
>
>     Best regards,
>     --aryeh
>
>
>
>             Hi Aryeh,
>
>             Thanks for following up on the ImageJ list.
>
>             For reference:
>             - the closed ticket[1]
>             - commit on develop[2] (Bio-Formats 5.1.x)
>
>                 On the OME tracking system, this bug is said to be
>                 resolved. If so, then
>
>             the corrected bioformats is not yet distributed on the
>             fiji update sites.
>
>             Fiji uses Bio-Formats 5.0.x, with the dev version[3]
>             available from the
>             Bio-Formats development update site[4]. This fix will
>             become part of core
>             Fiji when there's a 5.1.0 release.
>
>                 I also downloaded the appropriate jar files from
>                 ci.openmicroscopy.org/view/Bio-Formats/job/BIOFORMATS-5.1-latest/lastSuccessfulBuild/artifact/artifacts/
>                 <http://ci.openmicroscopy.org/view/Bio-Formats/job/BIOFORMATS-5.1-latest/lastSuccessfulBuild/artifact/artifacts/>
>
>             <http://ci.openmicroscopy.org/view/Bio-Formats/job/BIOFORMATS-5.1-latest/lastSuccessfulBuild/artifact/artifacts/>
>
>             I believe this was the correct place to pull artifacts
>             from to test the ND2
>             fix, but you should not use 5.0.x jars with 5.1.x. If you
>             want to try
>             5.1.x, you should replace everything in
>             Fiji.app/jars/bio-formats with its
>             5.1.x equivalent.
>
>             Hope that helps. Good luck!
>
>             Best,
>             Mark
>
>             [1] http://trac.openmicroscopy.org.uk/ome/ticket/12548
>             [2]
>             https://github.com/openmicroscopy/bioformats/commit/6e6387f8206889b416fe4df9f61d72f782ca0e9e
>             [3] https://github.com/openmicroscopy/bioformats/tree/dev_5_0
>             [4] http://imagej.net/List_of_update_sites
>
>             --
>             ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>         Dr. David Knecht
>         Professor of Molecular and Cell Biology
>         Core Microscopy Facility Director
>         University of Connecticut
>         Storrs, CT 06269
>         860-486-2200 <tel:860-486-2200>
>
>         --
>         ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>
>
>

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051


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