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Re: 32-Bit Tiff Single Pixel Noise Removal

Posted by Francis on Feb 05, 2015; 6:42pm
URL: http://imagej.273.s1.nabble.com/32-Bit-Tiff-Single-Pixel-Noise-Removal-tp5011416p5011461.html

Michael,

Thank you for your suggestion. When I look at the data in log view it
is exactly what I would expect. But the pixels that are distorting my view
need to be highlighted and removed as instrument artifacts. Any ideas?

Best,

Francis

On Tue, Feb 3, 2015 at 9:16 AM, Michael Schmid <[hidden email]>
wrote:

> Hi Francis,
>
> it depends what you want to do with the images. If the pixel intensities
> are not important (e.g. you only want to detect the position of the
> elliptical areas) the easiest would be using the logarithm of the image
> (Process>Math>Log).
>
> I don't think that the excessively high pixel values are noise.
> Essentially all of the structure in the image seems to be made of streaky
> features similar to the very bright 'noise'.
>
> Michael
> ________________________________________________________________
> On Feb 3, 2015, at 03:26, Francis OBrien wrote:
>
> > Hi,
> >
> > I need to remove single pixel noise from 1000's of 32-bit TIFF images. I
> tried to use the outlier process but its not working as I have to manually
> set the threshold. I think I need to automatically apply a known 95 or 99%
> threshold to the minimum and maximum of the histogram data before I can
> properly visualize the images.
> >
> > I am attaching an example of the type of image and its histogram. I hope
> someone on the list could advise on a batch type macro that could solve
> this problem.
> >
> > Best,
> >
> > Francis
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



--
Regards,

Francis

Research graduate,
New York School of Medicine
New York

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