Re: How to count cells in stacks? (tried 3D object counter)

Posted by Anda Cornea on Feb 06, 2015; 12:16am
URL: http://imagej.273.s1.nabble.com/How-to-count-cells-in-stacks-tried-3D-object-counter-tp5011466p5011468.html

I just happened upon this paper today and I thought it may help you:

 http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00027/full?utm_source=newsletter&utm_medium=email&utm_campaign=Neuroscience-w21-2014

Good luck!



-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of KerL
Sent: Thursday, February 05, 2015 2:05 PM
To: [hidden email]
Subject: How to count cells in stacks? (tried 3D object counter)

Hi all,

I have a stack of images that I would like to quantify. Some cells appear in more than 1 z-slice, therefore the possibility of counting a cell twice remains. Is there a macro that allows me to solve this problem, preferably one that allows me to use the regular ROI manager. I have tried using the 3D object counter plugin, but the 3D ROI manager does not allow me to look at the counted object in the original image.

<http://imagej.1557.x6.nabble.com/file/n5011466/Capture.png>



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