3D Viewer help - "stretched" stacked images

Posted by JoeG on Feb 21, 2015; 7:51pm
URL: http://imagej.273.s1.nabble.com/3D-Viewer-help-stretched-stacked-images-tp5011714.html

Hi all,

First time mailing list poster and relatively new user of ImageJ/Fiji here.

I am trying to use the 3D Viewer feature on stacked (.stk) files of yeast cells. When I do this it "separates" each slice as shown in the attached file. I cropped this image for simplification, but have the same result on all files. I have tried 3D Viewer with the sample images native to ImageJ/Fiji and have no problem, so I'm pretty sure the issue is with my files. I have also tried to "prep" the images in several ways by separating the stacked files, saving as .tiff or RGB, processing back to stacks/hyperstacks and always have the same results. Trying different numbers in the translation feature does not seem to make a difference.

All my drivers and updates are current.

Any advice is much appreciated.

Best,
Joe