Posted by G. Esteban Fernandez on Feb 28, 2015; 6:05pm URL: http://imagej.273.s1.nabble.com/free-open-source-deconvolution-tp5011779p5011816.html
I tested all the decon plugins and options available for ImageJ 1-2
years ago and, in my hands for my particular application, Piotr
Wendykier's Parallel Iterative Deconvolution plugin with the WPL
option and a measured PSF worked best
(https://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolution).
With that I routinely deconvolve 3D+time data taken through a 20x/0.4
LWD lens using a measured PSF cropped to 64x64x123.
AutoQuant AutoDeblur blind decon was better in my tests but it costs
money. MIPAV is free and apparently employs the same or a similar
blind decon algorithm
(http://mipav.cit.nih.gov/pubwiki/index.php/Microscopy:_Blind_Deconvolution)
but I remember that back then I couldn't get it to run. I have a
better computer now; I should try MIPAV again....
-Esteban
On Wed, Feb 25, 2015 at 8:48 PM, JOEL B. SHEFFIELD <[hidden email]> wrote:
> I have been impressed by the plugins that Bob Dougherty has written --one
> generates a theoretical PSF, and the other carries out iterative
> deconvolution. You can find them on the IJ website under his name. or,
> follow this link: http://www.optinav.com/imagej.html >
> Joel
>
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email] > URL: *http://tinyurl.com/khbouft <http://tinyurl.com/khbouft>*
>
> On Wed, Feb 25, 2015 at 11:24 PM, nikhil <[hidden email]> wrote:
>
>> Hi,
>>
>> i need to do a 3-d deconvolution of stacks of fluorescence widefield
>> images, apart from deconvolution lab by EPFL is there any open source
>> package available for deconvolution ? Is it possible to write the some
>> algorithms like richardson lucy in matlab for doing 3-d deconvolution ?
>>
>>
>>
>> --
>> View this message in context:
>> http://imagej.1557.x6.nabble.com/free-open-source-deconvolution-tp5011779.html >> Sent from the ImageJ mailing list archive at Nabble.com.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html >>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html