Macro for recognize shapes.

Posted by Brioso Jimenez, Jose on Mar 02, 2015; 10:30am
URL: http://imagej.273.s1.nabble.com/Macro-for-recognize-shapes-tp5011825.html

Hello everyone!


I am trying to use ImageJ to measure the fluorescence of a transgenic strain of C. elegans that harbours both GFP and RFP, for that I am taking multiple pictures of them in different conditions, with a lot of replicas.


Because I have SO MUCH data I need to find a way that let ImageJ recognize the shape of the worms so I can measure the fluorescence per area with ImageJ.


I am taking three pictures per measurement, one without filters, one with GFP filter and the last one with RFP filter. (Pics attached)


How can I do to develop a macro that allow ImageJ to recognize the shapes so I can measure the fluorescence, and I do not have to draw of the worm manually (that is painfull).

?
Thank you all for your time!

Best regards.

JL. Brioso?

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