Re: Macro for recognize shapes.

Posted by G. Esteban Fernandez on Mar 02, 2015; 2:29pm
URL: http://imagej.273.s1.nabble.com/Macro-for-recognize-shapes-tp5011825p5011828.html

1. Image > Adjust > Threshold (to select the worms)
2. Analyze > Set Measurements and check the box for Mean Gray Value
and/or Integrated Density.
3. Analyze > Analyze Particles.

-Esteban

On Mon, Mar 2, 2015 at 2:30 AM, Brioso Jimenez, Jose
<[hidden email]> wrote:

> Hello everyone!
>
>
> I am trying to use ImageJ to measure the fluorescence of a transgenic strain of C. elegans that harbours both GFP and RFP, for that I am taking multiple pictures of them in different conditions, with a lot of replicas.
>
>
> Because I have SO MUCH data I need to find a way that let ImageJ recognize the shape of the worms so I can measure the fluorescence per area with ImageJ.
>
>
> I am taking three pictures per measurement, one without filters, one with GFP filter and the last one with RFP filter. (Pics attached)
>
>
> How can I do to develop a macro that allow ImageJ to recognize the shapes so I can measure the fluorescence, and I do not have to draw of the worm manually (that is painfull).
>
> ?
> Thank you all for your time!
>
> Best regards.
>
> JL. Brioso?
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html