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How to measure nuclear protein spatial distribution

Posted by PEARSON Matthew on Mar 02, 2015; 3:45pm
URL: http://imagej.273.s1.nabble.com/Macro-for-recognize-shapes-tp5011825p5011830.html

Hi all,

One of our facility users would like to put some numbers on the distribution of their nuclear protein in 2D which in some cells localises more to the periphery and in others is more randomly distributed.  Is there an easy way to quantify this?  Ideally by segmenting the entire nucleus then using some parameter to measure the intensity and distribution within.

HIstorically, here we have divided the nucleus into 5 concentric bands each with equal area and then measured the intensity within each ring as a means to be able to compare distribution between different nuclei.

Thanks for the help,

Matt






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