Problems with Analyze particles

Posted by Maria Smedh on
URL: http://imagej.273.s1.nabble.com/Problems-with-Analyze-particles-tp5011843.html

Hi,

I have just realized that my objects get too large when segmenting a fluorescent image in Fiji. I threshold the image and then use "Analyze particles" to create the segmented objects. My problem is that "Analyze particles" always fills holes, no matter if I have ticked the box "Include holes" or not!

I first tried to upgrade to the latest version of Fiji and then I tried to install a Life-line version, but I get the same results anyways. I have also tried with ImageJ and on a PC (my computer is a MacBook Pro).

Can anyone help me?

Best regards, Maria

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MARIA SMEDH, PhD



Centre for Cellular Imaging
Core Facilities, the Sahlgrenska Academy
Univ. of Gothenburg, Sweden



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