Re: Problems with Analyze particles

Posted by gankaku on
URL: http://imagej.273.s1.nabble.com/Problems-with-Analyze-particles-tp5011843p5011849.html

Hi Maria,

can you potentiall post an binary example image with which you detect this
problem. This will make it easier to figure out what might be the problem.
In the current Fiji version I do not see any problem with the Analyze
Particle function if holes are in some particles.

best,
Jan

2015-03-03 16:51 GMT+01:00 Maria Smedh <[hidden email]>:

> Hi,
>
> I have just realized that my objects get too large when segmenting a
> fluorescent image in Fiji. I threshold the image and then use "Analyze
> particles" to create the segmented objects. My problem is that "Analyze
> particles" always fills holes, no matter if I have ticked the box "Include
> holes" or not!
>
> I first tried to upgrade to the latest version of Fiji and then I tried to
> install a Life-line version, but I get the same results anyways. I have
> also tried with ImageJ and on a PC (my computer is a MacBook Pro).
>
> Can anyone help me?
>
> Best regards, Maria
>
> ------------------------------
> MARIA SMEDH, PhD
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> Centre for Cellular Imaging
> Core Facilities, the Sahlgrenska Academy
> Univ. of Gothenburg, Sweden
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