TrakEM2: Stitching overlapping images from coordinates

Posted by André Pereira on Mar 09, 2015; 3:26am
URL: http://imagej.273.s1.nabble.com/TrakEM2-Stitching-overlapping-images-from-coordinates-tp5011918.html

Hi everybody,

We are trying to stitch a very large dataset (~90GB) of overlapping images. We used the Grid/collection stitching plugin, which was able to stitch the images correctly in smaller datasets, but we ran into memory problems with the larger ones. We then switched to TrakEM2 which is doing a great job at handling the large ones.

We are, however, facing a problem with the positioning of the tiles. After importing the images using a text file with the coordinates, the tiles are not positioned in the exact position. There is a small, but important shift. We verified our coordinates in the text file and they are correct. Our reconstructions with the Grid/collection stitching plugin, for instance, are accurate.

Has anyone had this problem before?

Thanks for your help!

Kind regards,
Andre Pereira

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