Posted by
Albert Cardona-2 on
Mar 09, 2015; 7:25pm
URL: http://imagej.273.s1.nabble.com/TrakEM2-Stitching-overlapping-images-from-coordinates-tp5011918p5011931.html
Andre,
Is there a calibration issue? Please could you post a few lines of the TXT
file for loading tiles, the expected positions, and the actual positions.
To obtain the actual positions, select them all and run this jython script:
from ini.trakem2.display import Display
for patch in Display.getSelected():
print patch.getAffineTransform()
Best,
Albert
2015-03-08 23:26 GMT-04:00 Andre Pereira <
[hidden email]>:
> Hi everybody,
>
> We are trying to stitch a very large dataset (~90GB) of overlapping
> images. We used the Grid/collection stitching plugin, which was able to
> stitch the images correctly in smaller datasets, but we ran into memory
> problems with the larger ones. We then switched to TrakEM2 which is doing a
> great job at handling the large ones.
>
> We are, however, facing a problem with the positioning of the tiles. After
> importing the images using a text file with the coordinates, the tiles are
> not positioned in the exact position. There is a small, but important
> shift. We verified our coordinates in the text file and they are correct.
> Our reconstructions with the Grid/collection stitching plugin, for
> instance, are accurate.
>
> Has anyone had this problem before?
>
> Thanks for your help!
>
> Kind regards,
> Andre Pereira
>
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