Posted by Jan Eglinger on Mar 17, 2015; 3:40pm URL: http://imagej.273.s1.nabble.com/Measure-channel-shift-in-subpixel-resolution-tp5012015.html
Hi all,
I am trying to measure the (unavoidable) shift between two color
channels using a 3D stack of fluorescent beads acquired at the different
channels.
As far as I can see, the Feature Extraction plugins (SIFT [1], MOPS [2]
and block matching [3]) only work on a single slice (2D).
In the SPIM_Registration / bigdataviewer plugins, there are algorithms
to detect 3D feature correspondences, but I'm not sure how to access
them by scripting.
What I would like to extract is the 3D translation matrix between two
stacks containing the image data of fluorescent beads, so that it can be
applied to other images using TransformJ for example.
Is there an easy way to get at that?