problem with the graph after calibration

Posted by Ian Evans on Mar 24, 2015; 5:28pm
URL: http://imagej.273.s1.nabble.com/problem-with-the-graph-after-calibration-tp5012146.html

I use imageJ mainly for quantification of westerns. After I input the OD values of an optical density step tablet (see first screenshot)I use the Redbird function to get a calibration curve, however the graph doesn’t display correctly even though the equation of the line seems reasonable (2nd screenshot). Is this a known bug or am I doing something wrong?

Thanks




Dr Ian Evans
Centre for Cardiovascular Biology & Medicine
Department of Medicine
Rayne Building
UCL Medical School
5 University Street
London
WC1E 6JJ

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