Help: setting a scale - not in the simple way!

Posted by Rotem Oved on Mar 25, 2015; 5:33am
URL: http://imagej.273.s1.nabble.com/Help-setting-a-scale-not-in-the-simple-way-tp5012156.html

hello everybody,i'm trying to measure length using Fiji. However, the images created by the microscope do not have a scale bar in them. Is there a way I can set the scale properly, converting pixels into nm?I tried using "Bio-Formats Importer" to open the image, but still, the length shown in "all paths" window doesn't match the length that shows up after performing the "analyze skeleton" (I added a photo for example).I'm sure I can retrieve the information because it does appear (hopefully, the correct data) at the "all paths" window.thank you so much.

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