Thanks Michael it was very helpful, but i also want to eliminate the background. I want to have only the ROI in the image without any backgroound. Is it possible to do in imagej?
> Date: Wed, 25 Mar 2015 16:05:59 +0100
> From:
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> Subject: Re: manipulating ROI
> To:
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>
> On Mar 25, 2015, at 15:46, rabih assaf wrote:
>
> > HOw to extract a ROI withoud the surrounding regions? (the duplicate don't do the work)
> > thanks in advance
>
> Hi Rabih,
>
> if you want extract only the contents of a non-rectangular selection, you can use
>
> (1) Duplicate and Clear Outside; then the outside becomes the background color. You can define the beckground color when you double-cklick the Eyedropper tool, click the "Reverse Foreground&Background" double arrow, select the color and reverse foreground&background again. Also the small symbol with black&white rectangles at bottom right can be of use.
>
> (2) Duplicate, Edit>Selection>Make Inverse and Process>Math>Set to the desired backgorund value, or
>
> (3) Copy and Paste into an new window.
>
> Michael
>
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