Posted by
bnorthan on
Mar 31, 2015; 6:26pm
URL: http://imagej.273.s1.nabble.com/Cell-counting-tp5012266p5012274.html
Hi Jenny
Under the "Image->Adjust" menu there is a "color threshold..." plugin.
This brings up a dialog that gives you the means to try thresholds in
different color spaces. There is even a button to export to a macro.
There is also a plugin called 'Color Inspector 3D'. This displays the
pixel values in a 3D color cube. It also has the option to use different
color spaces.
Once you have something that seems to work on a few representative images
you could record it as a macro. That's not always straight forward but the
list can probably help you with questions about that.
On Tue, Mar 31, 2015 at 1:48 PM, veraelisabeth <
[hidden email]>
wrote:
> Hi Jenny,
>
> I'm relatively new to imagej and I see some problems with it, regarding the
> subjectivity of it. But I'd try to separate the color channels (although I
> don't know if thats possible with your sort of image (I use
> immunofluorescence of cell cultures and it's possible)
>
> Then I would download the color pixel counter. Afterwards you could
> calculate all coloured pixels / mean pixels of one cell to get the number
> of
> cells
>
> But as I said. I'm quite new to imagej
>
>
>
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