new to image j

Posted by Godfrey Pasirayi on Apr 01, 2015; 9:55am
URL: http://imagej.273.s1.nabble.com/new-to-image-j-tp5012294.html

Hello  I am in the process of completing my research and part of my work requires the analysis of images using Image J. The problem I have is to create a small macro that I can using to analyse a ROI in a  number of images. Can someone in the group assist me to get around this problem as a new user of image J. Secondly, when I try to find the logarithmic relationship between two images A and B ( Log10 (A/B))     the resultant image C is completely dark. My question is that,  Is there any scaling that needs to be done  so that I can see the image C clearly. I have tried to effect about 0.25% of enhanced contrast and the image is visible but not very clear.
  ( Log10 (A/B)) was obtained by selecting  Process - Image calculator -  A ( Blank SP) divide  B ( SP 0.6) and checking 32-bit - Math - Log -Math - dividing by 2.303 to give image C.  I wanted to attach the Pictures A and B but they have been rejected  Thank you so much for assistance. regards Godfrey

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html