Re: bUnwarpJ for Z-stack fluorescence image

Posted by Ignacio Arganda-Carreras-2 on Apr 01, 2015; 2:33pm
URL: http://imagej.273.s1.nabble.com/bUnwarpJ-for-Z-stack-fluorescence-image-tp5012280p5012298.html

Hello John,

bUnwarpJ can correct misalignments in 2D but remember it's not a 3D
registratoin method so it won't affect the Z axis of your data.

What do you exactly need to register? A sequence of 2D images?

ignacio

On Wed, Apr 1, 2015 at 4:34 AM, LIM Soon Yew John (IMB) <
[hidden email]> wrote:

> Dear All,
>
> Can I please check if bUnwarpJ can be used to correct for chromatic
> aberration for 2 channels Z-stack super-resolution fluorescence images?
> Will the transformation cause any misalignment in Z axis?
>
> Please note the transformation file for bUnwarpJ is created by using
> super-resolution images from 0.1 um multi-wavelength beads.
>
> Best Regards,
> John
>
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>

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