Re: LSM Split Channels Broken

Posted by Ignacio Arganda-Carreras-2 on Apr 02, 2015; 8:20am
URL: http://imagej.273.s1.nabble.com/LSM-Split-Channels-Broken-tp5012281p5012310.html

Hello Rebecca,

I observed the same problems.

The channel splitting can be fixed if you convert the image first to a
Composite (in a macro run("Make Composite", "display=Composite"); ). But I
agree it shouldn't be like this.

ignacio

On Thu, Apr 2, 2015 at 1:35 AM, Rebecca Keller <[hidden email]> wrote:

> A follow-up to this problem: for some reason, FIJI thinks my images are
> interleaved rather than separate channels, even though they appear with a
> channel-slider--I've been able to separate the channels by using
> "deinterleave" rather than "split channels." I think this is probably a
> bona fide bug...
>
> JPK
>
> On Tue, Mar 31, 2015 at 9:51 PM, Rebecca Keller <[hidden email]>
> wrote:
>
> > Dear Imagers,
> >
> > Two things have recently happened with my LSM files:
> >
> > -I now need to import rather than just opening them
> >
> > -Split channels does not work; complains with "multichannel image
> > required." (But my images are multichannel, and I can see both with the
> > slider.)
> >
> > Neither of these was a problem before--any thoughts as to what's going
> on?
> >
> > JPK
> >
>
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>

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Ignacio Arganda-Carreras, Ph.D.
Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech
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